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rabbit polyclonal anti integrin β5  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit polyclonal anti integrin β5
    Rabbit Polyclonal Anti Integrin β5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+integrin+%CE%B25/Integrin+beta5+Antibody/pmc12042775-246-159-166
    Average 93 stars, based on 49 article reviews
    rabbit polyclonal anti integrin β5 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Western Blot:

    Article Title: Extracellular vesicles adhere to cells primarily by interactions of integrins and GM1 with laminin
    Article Snippet: .. The following primary antibodies were used for western blotting: mouse monoclonal anti-β actin 15G5A11/E2 (1:5,000, Cat# MA1–140; Thermo Fisher Scientific), mouse monoclonal anti-CD63 8A12 (1:1,000, Cat# SHI-EXO-M02; Cosmo Bio), mouse monoclonal anti-CD81 B11 (1:500, Cat# SC166029; Santa Cruz Biotechnology), rabbit monoclonal anti-CD9 EPR23105-125 (1:1,000, Cat# ab263019; Abcam), mouse monoclonal anti-CD151 11G5a (1:250, Cat# ab33315; Abcam), rabbit polyclonal anti-fibronectin (1:1,000, Cat# F3648; Sigma-Aldrich), rabbit monoclonal anti-GAPDH 14C10 (1:2,000, Cat# 2181S; Cell Signaling), rabbit monoclonal anti-integrin α2 EPR5788 (1:2,500, Cat# ab133557; Abcam), rabbit polyclonal anti-integrin α3 (1:500, Cat# ab190731; Abcam), rabbit polyclonal anti-integrin α5 (1:250, Cat# 4705S; Cell Signaling), rabbit monoclonal anti-integrin α5 clone EPR7854 (1:500, Cat# ab150361; Abcam), rabbit polyclonal anti-integrin α6 (1:500, Cat# 3750S; Cell Signaling), rabbit polyclonal anti-integrin α7 (1:500, Cat# ab203254; Abcam), rabbit polyclonal anti-integrin αV (1:500, Cat# ab117611; Abcam), mouse monoclonal anti-CD29 Clone 18 (1:2,000, Cat# 610467; BD Bioscience), rabbit polyclonal anti-integrin β3 (1:500, Cat# AB2984; Millipore), rabbit polyclonal anti-integrin β4 (1:500, Cat# 4707S; Cell Signaling), rabbit polyclonal anti-integrin β5 (1:500, Cat# 4708S; Cell Signaling), rabbit polyclonal anti-laminin 1+2 (1:1,000, Cat# ab7463; Abcam), mouse monoclonal anti-laminin clone 2E8 (1:500, Cat# MAB1920; Millipore), mouse monoclonal anti-talin-1 97H6 (1:500, Cat# GTX38972; Gene Tex), rabbit polyclonal anti-phospho-talin (Ser425), (1:250, Cat# TP4171; ECM Biosciences), mouse monoclonal anti–kindlin-2 clone 3A3 (1:500, Cat# MAB2617; Sigma-Aldrich), and anti-GM3 antibody clone GMR6 (1:1,000, Cat# A2582; Tokyo Chemical Industry), along with biotin-conjugated cholera toxin B subunit (CTXB; 1:500, Cat# 112; List Labs). .. Additionally, goat anti-mouse IgG-HRP (1:5,000, Cat# 12–349; Millipore), donkey anti-rabbit IgG-HRP (1:4,000, Cat# NA934; Cytiva), goat anti-rabbit IgG-HRP (1:10,000, Cat# A0545; Sigma-Aldrich), goat anti-mouse IgM antibody-HRP (1:10,000, Cat# 31440; Invitrogen), and rabbit anti-biotin antibody-HRP clone D5A7 (1:10,000, Cat# 5571; Cell Signaling) were used as secondary antibodies.

    Article Title: Extracellular vesicles adhere to cells primarily by interactions of integrins and GM1 with laminin.
    Article Snippet: .. The following primary antibodies were used for western blotting: mouse monoclonal anti-β actin 15G5A11/E2 (1:5,000, Cat# MA1–140; Thermo Fisher Scientific), mouse monoclonal antiCD63 8A12 (1:1,000, Cat# SHI-EXO-M02; Cosmo Bio), mouse monoclonal anti-CD81 B11 (1:500, Cat# SC166029; Santa Cruz Biotechnology), rabbit monoclonal anti-CD9 EPR23105-125 (1: 1,000, Cat# ab263019; Abcam), mouse monoclonal anti-CD151 11G5a (1:250, Cat# ab33315; Abcam), rabbit polyclonal antifibronectin (1:1,000, Cat# F3648; Sigma-Aldrich), rabbit monoclonal anti-GAPDH 14C10 (1:2,000, Cat# 2181S; Cell Signaling), rabbit monoclonal anti-integrin α2 EPR5788 (1:2,500, Cat# ab133557; Abcam), rabbit polyclonal anti-integrin α3 (1:500, Cat# ab190731; Abcam), rabbit polyclonal anti-integrin α5 (1:250, Cat# 4705S; Cell Signaling), rabbit monoclonal anti-integrin α5 clone EPR7854 (1:500, Cat# ab150361; Abcam), rabbit polyclonal anti-integrin α6 (1:500, Cat# 3750S; Cell Signaling), rabbit polyclonal anti-integrin α7 (1:500, Cat# ab203254; Abcam), rabbit polyclonal anti-integrin αV (1:500, Cat# ab117611; Abcam), mouse monoclonal anti-CD29 Clone 18 (1:2,000, Cat# 610467; BD Bioscience), rabbit polyclonal anti-integrin β3 (1:500, Cat# AB2984; Millipore), rabbit polyclonal anti-integrin β4 (1:500, Cat# 4707S; Cell Signaling), rabbit polyclonal anti-integrin β5 (1: 500, Cat# 4708S; Cell Signaling), rabbit polyclonal anti-laminin 1+2 (1:1,000, Cat# ab7463; Abcam), mouse monoclonal antilaminin clone 2E8 (1:500, Cat# MAB1920; Millipore), mouse monoclonal anti-talin-1 97H6 (1:500, Cat# GTX38972; Gene Tex), rabbit polyclonal anti-phospho-talin (Ser425), (1:250, Cat# TP4171; ECM Biosciences), mouse monoclonal anti–kindlin-2 clone 3A3 (1:500, Cat# MAB2617; Sigma-Aldrich), and anti-GM3 antibody clone GMR6 (1:1,000, Cat# A2582; Tokyo Chemical Industry), along with biotin-conjugated cholera toxin B subunit (CTXB; 1:500, Cat# 112; List Labs). .. Additionally, goat anti-mouse IgG-HRP (1:5,000, Cat# 12–349; Millipore), donkey anti-rabbit IgG-HRP (1:4,000, Cat# NA934; Cytiva), goat anti-rabbit IgGHRP (1:10,000, Cat# A0545; Sigma-Aldrich), goat anti-mouse IgM antibody-HRP (1:10,000, Cat# 31440; Invitrogen), and rabbit anti-biotin antibody-HRP clone D5A7 (1:10,000, Cat# 5571; Cell Signaling) were used as secondary antibodies.



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    Quantitative real-time PCR for selected genes (IL1A, DUSP1, COL1A1 and ITGB5) found to be differentially expressed in gene microarrays. Both in ES-2 cells and Caov-3 cells, the relative mRNA expression of 4 genes was significantly higher in HE4 high expressed cells and lower in HE4 low expressed cells (panel A, compared with Mock cells, one-way ANOVA). ICC staining showed that integrin β5 was highly expressed in HE4-high expression cells, relatively low expressed in the mock cells (HE4-H-Mock and HE4-L-Mock) and sparsely expressed in HE4 low expression cells (panel B) Immunohistochemical staining in xenograft tumor tissues showed that integrin β5 was high expressed in all the HE4 highly expressed tumor tissues, relatively low expressed in the HE4-H-Mock tumors and totally not expressed in HE4-L tumors (100%, 80% and 0% in 3 groups, respectively, P=0.003, panel C). IHC staining of ovarian cancer sample showed that expressions of HE4 and integrin β5 were mainly on membrane and cytoplasm, their expressions were relatively lower in chemotherapy sensitive group than the resistant group, the correlation coefficient was 0.213 (Spearman correlation analysis, P = 0.042, panel D). Kaplan-Meier survival analysis (panel E) showed that high expressions of HE4 and integrin β5 were independent risk factors for overall survival (OS) and progression-free survival (PFS), log Rank P = 0.001, 0.001 for HE4 in OS and PFS, P = 0.029, 0.042 for integrin β5 in OS and PFS, respectively.

    Journal: Oncotarget

    Article Title: Overexpression of HE4 (human epididymis protein 4) enhances proliferation, invasion and metastasis of ovarian cancer

    doi:

    Figure Lengend Snippet: Quantitative real-time PCR for selected genes (IL1A, DUSP1, COL1A1 and ITGB5) found to be differentially expressed in gene microarrays. Both in ES-2 cells and Caov-3 cells, the relative mRNA expression of 4 genes was significantly higher in HE4 high expressed cells and lower in HE4 low expressed cells (panel A, compared with Mock cells, one-way ANOVA). ICC staining showed that integrin β5 was highly expressed in HE4-high expression cells, relatively low expressed in the mock cells (HE4-H-Mock and HE4-L-Mock) and sparsely expressed in HE4 low expression cells (panel B) Immunohistochemical staining in xenograft tumor tissues showed that integrin β5 was high expressed in all the HE4 highly expressed tumor tissues, relatively low expressed in the HE4-H-Mock tumors and totally not expressed in HE4-L tumors (100%, 80% and 0% in 3 groups, respectively, P=0.003, panel C). IHC staining of ovarian cancer sample showed that expressions of HE4 and integrin β5 were mainly on membrane and cytoplasm, their expressions were relatively lower in chemotherapy sensitive group than the resistant group, the correlation coefficient was 0.213 (Spearman correlation analysis, P = 0.042, panel D). Kaplan-Meier survival analysis (panel E) showed that high expressions of HE4 and integrin β5 were independent risk factors for overall survival (OS) and progression-free survival (PFS), log Rank P = 0.001, 0.001 for HE4 in OS and PFS, P = 0.029, 0.042 for integrin β5 in OS and PFS, respectively.

    Article Snippet: Rabbit polyclonal anti-HE4 antibody and rabbit polyclonal anti-integrin β5 antibody were purchased from Abcam Company (USA).

    Techniques: Real-time Polymerase Chain Reaction, Expressing, Staining, Immunohistochemical staining, Immunohistochemistry, Membrane

    Comparison of clinical characteristics and immunohistochemical expression of HE4 and  Integrin   β5  in 92 cases of epithelial ovarian cancer

    Journal: Oncotarget

    Article Title: Overexpression of HE4 (human epididymis protein 4) enhances proliferation, invasion and metastasis of ovarian cancer

    doi:

    Figure Lengend Snippet: Comparison of clinical characteristics and immunohistochemical expression of HE4 and Integrin β5 in 92 cases of epithelial ovarian cancer

    Article Snippet: Rabbit polyclonal anti-HE4 antibody and rabbit polyclonal anti-integrin β5 antibody were purchased from Abcam Company (USA).

    Techniques: Comparison, Immunohistochemical staining, Expressing

    Expression of HE4 and  integrin   β5  in chemotherapy sensitive group and resistant group of 92 cases of epithelial ovarian cancer

    Journal: Oncotarget

    Article Title: Overexpression of HE4 (human epididymis protein 4) enhances proliferation, invasion and metastasis of ovarian cancer

    doi:

    Figure Lengend Snippet: Expression of HE4 and integrin β5 in chemotherapy sensitive group and resistant group of 92 cases of epithelial ovarian cancer

    Article Snippet: Rabbit polyclonal anti-HE4 antibody and rabbit polyclonal anti-integrin β5 antibody were purchased from Abcam Company (USA).

    Techniques: Expressing